TG006585

Peptide	R.VYCN[H9N2]LSAAHTFEDLK.N (PE002193)
Sequence	VYCNLSAAHTFEDLK
Glycans(s)	H9N2 (N4)
Mod(s)	57.021 (C3)
Molecular Weight	3,649.474
Charge	3+
Protein	PXDN (Q92626)
Spectrum File	MS_52_HEK_secretome01
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction
Sample	HEK293 Cells

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
20.487	20.441	29.951	1,211.500	3+	985.969	2+	Y[pN]	100.0
20.487	20.441	29.951	1,211.500	3+	1,411.614	2+	Y[pH4N2]	48.4
20.487	20.441	29.951	1,211.500	3+	1,573.667	2+	Y[pH6N2]	43.6
20.487	20.441	29.951	1,211.500	3+	1,654.693	2+	Y[pH7N2]	36.7
20.487	20.441	29.951	1,211.500	3+	1,168.535	2+	Y[pHN2]	36.1
20.487	20.441	29.951	1,211.500	3+	1,492.640	2+	Y[pH5N2]	35.6
20.487	20.441	29.951	1,211.500	3+	1,249.561	2+	Y[pH2N2]	35.3
20.487	20.441	29.951	1,211.500	3+	1,087.508	2+	Y[pN2]	33.2
20.487	20.441	29.951	1,211.500	3+	1,103.465	3+	Y[pH7N2]	31.7
20.487	20.441	29.951	1,211.500	3+	1,330.588	2+	Y[pH3N2]	28.8
20.487	20.441	29.951	1,211.500	3+	1,049.447	3+	Y[pH6N2]	26.2
20.487	20.441	29.951	1,211.500	3+	1,157.482	3+	Y[pH8N2]	21.7
20.487	20.441	29.951	1,211.500	3+	995.430	3+	Y[pH5N2]	17.7
20.487	20.441	29.951	1,211.500	3+	941.412	3+	Y[pH4N2]	15.0
20.487	20.441	29.951	1,211.500	3+	779.359	3+	Y[pHN2]	9.5
20.487	20.441	29.951	1,211.500	3+	887.394	3+	Y[pH3N2]	9.2
20.487	20.441	29.951	1,211.500	3+	833.377	3+	Y[pH2N2]	8.7
20.487	20.441	29.951	1,211.500	3+	1,970.930	1+	Y[pN]	8.1
20.487	20.441	29.951	1,211.500	3+	725.342	3+	Y[pN2]	6.5

Spectra

4139;EThcD;20.480;4130;1211.827;Byonic:q-value=2.55e-06/GPS:MinICScore=143.153
4140;HCD;20.487;4130;1211.827;