TG004838

Peptide	K.SIGLLSPDFQEDN[H4N4FS]ETEINFLLK.Q (PE001751)
Sequence	SIGLLSPDFQEDNETEINFLLK
Glycans(s)	H4N4FS (N13)
Mod(s)
Molecular Weight	4,436.951
Charge	3+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
66.979	67.207	133.826	1,473.988	3+	1,363.177	2+	Y[pN]	31.5
66.979	67.207	133.826	1,473.988	3+	1,882.364	2+	Y[pH3N3F]	14.6
66.979	67.207	133.826	1,473.988	3+	1,436.206	2+	Y[pNF]*	11.8
66.979	67.207	133.826	1,473.988	3+	1,626.769	2+	Y[pH2N2]	10.1
66.979	67.207	133.826	1,473.988	3+	1,809.336	2+	Y[pH3N3]	9.1
66.979	67.207	133.826	1,473.988	3+	1,801.338	2+	Y[pH2N3F]	7.3
66.979	67.207	133.826	1,473.988	3+	1,464.717	2+	Y[pN2]	7.1
66.979	67.207	133.826	1,473.988	3+	1,728.309	2+	Y[pH2N3]	6.1
66.979	67.207	133.826	1,473.988	3+	1,545.743	2+	Y[pHN2]	6.0
66.979	67.207	133.826	1,473.988	3+	1,707.796	2+	Y[pH3N2]	5.8
66.979	67.207	133.826	1,473.988	3+	1,780.825	2+	Y[pH3N2F]*	5.7
66.979	67.207	133.826	1,473.988	3+	1,699.798	2+	Y[pH2N2F]*	5.5

Spectra

13508;EThcD;66.784;13505;1473.997;Byonic:q-value=0.00434/GPS:MinICScore=65.487
13510;HCD;66.793;13505;1473.997;
13592;EThcD;67.158;13589;1473.997;Byonic:q-value=6.56e-08/GPS:MinICScore=13.038
13593;HCD;67.164;13589;1473.997;