TG004731

Peptide	K.LQLQALQQN[H5N4F2S]GSSVLSEDKSK.R (PE001721)
Sequence	LQLQALQQNGSSVLSEDKSK
Glycans(s)	H5N4F2S (N9)
Mod(s)
Molecular Weight	4,395.942
Charge	3+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin_2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
29.372	29.120	38.118	1,460.318	3+	1,362.946	3+	Y[pH5N4S]	40.3
29.372	29.120	38.118	1,460.318	3+	1,788.831	2+	Y[pH4N3F]	30.3
29.372	29.120	38.118	1,460.318	3+	1,707.804	2+	Y[pH3N3F]	21.5
29.372	29.120	38.118	1,460.318	3+	1,261.646	2+	Y[pNF]*	20.9
29.372	29.120	38.118	1,460.318	3+	1,314.601	3+	Y[pH5N4F]	18.3
29.372	29.120	38.118	1,460.318	3+	1,861.350	2+	Y[pH4N3S]	17.6
29.372	29.120	38.118	1,460.318	3+	1,188.617	2+	Y[pN]	14.6
29.372	29.120	38.118	1,460.318	3+	1,715.802	2+	Y[pH4N3]	13.7
29.372	29.120	38.118	1,460.318	3+	1,634.775	2+	Y[pH3N3]	12.2
29.372	29.120	38.118	1,460.318	3+	1,525.238	2+	Y[pH2N2F]*	10.9
29.372	29.120	38.118	1,460.318	3+	1,606.265	2+	Y[pH3N2F]*	10.6
29.372	29.120	38.118	1,460.318	3+	1,780.323	2+	Y[pH3N3S]	9.7
29.372	29.120	38.118	1,460.318	3+	1,452.209	2+	Y[pH2N2]	8.4
29.372	29.120	38.118	1,460.318	3+	1,533.236	2+	Y[pH3N2]	6.6
29.372	29.120	38.118	1,460.318	3+	1,934.379	2+	Y[pH4N3FS]	5.7
29.372	29.120	38.118	1,460.318	3+	1,265.915	3+	Y[pH5N4]	5.3
29.372	29.120	38.118	1,460.318	3+	1,290.157	2+	Y[pN2]	5.1

Spectra

5399;EThcD;29.372;5390;1460.328;Byonic:q-value=3.64e-05/GPS:MinICScore=1.405
5400;HCD;29.377;5390;1460.328;