TG004367

Peptide	R.EGVFVSN[H3N6F]GTHWFVTQR.N (PE001661)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H3N6F (N7)
Mod(s)
Molecular Weight	3,731.61
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.025	32.154	45.523	1,238.874	3+	1,756.268	2+	Y[pH3N5F]	54.5
32.025	32.154	45.523	1,238.874	3+	1,654.728	2+	Y[pH3N4F]	18.9
32.025	32.154	45.523	1,238.874	3+	1,573.702	2+	Y[pH2N4F]	15.6
32.025	32.154	45.523	1,238.874	3+	1,472.162	2+	Y[pH2N3F]	9.9
32.025	32.154	45.523	1,238.874	3+	1,683.239	2+	Y[pH3N5]	7.7
32.025	32.154	45.523	1,238.874	3+	1,581.699	2+	Y[pH3N4]	6.9
32.025	32.154	45.523	1,238.874	3+	1,399.133	2+	Y[pH2N3]	5.9

Spectra

6247;EThcD;31.951;6238;1239.213;Byonic:q-value=0.00632/GPS:MinICScore=18.941
6248;HCD;31.957;6238;1239.213;
6275;EThcD;32.076;6265;1238.883;Byonic:q-value=0.00104/GPS:MinICScore=12.951
6277;HCD;32.094;6265;1238.883;