TG004365

Peptide	R.EGVFVSN[H5N3F]GTHWFVTQR.N (PE001649)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N3F (N7)
Mod(s)
Molecular Weight	3,446.477
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.242	32.269	45.810	1,143.830	3+	1,532.675	2+	Y[pH4N2F]	75.1
32.242	32.269	45.810	1,143.830	3+	1,451.649	2+	Y[pH3N2F]	41.2
32.242	32.269	45.810	1,143.830	3+	1,459.646	2+	Y[pH4N2]	30.5
32.242	32.269	45.810	1,143.830	3+	1,378.620	2+	Y[pH3N2]	25.0
32.242	32.269	45.810	1,143.830	3+	1,613.701	2+	Y[pH5N2F]*	22.1
32.242	32.269	45.810	1,143.830	3+	1,034.001	2+	Y[pN]	17.3
32.242	32.269	45.810	1,143.830	3+	1,297.593	2+	Y[pH2N2]	16.5
32.242	32.269	45.810	1,143.830	3+	1,370.622	2+	Y[pH2N2F]	14.3
32.242	32.269	45.810	1,143.830	3+	1,107.030	2+	Y[pNF]	11.3
32.242	32.269	45.810	1,143.830	3+	1,216.567	2+	Y[pHN2]	8.3
32.242	32.269	45.810	1,143.830	3+	1,135.540	2+	Y[pN2]	7.8
32.242	32.269	45.810	1,143.830	3+	1,289.596	2+	Y[pHN2F]	6.8
32.242	32.269	45.810	1,143.830	3+	1,208.569	2+	Y[pN2F]	6.0
32.242	32.269	45.810	1,143.830	3+	1,540.672	2+	Y[pH5N2]*	5.4
32.242	32.269	45.810	1,143.830	3+	1,089.812	3+	Y[pH4N3F]	5.0

Spectra

6295;EThcD;32.174;6288;1143.838;Byonic:q-value=0.000176/GPS:MinICScore=13.792
6297;HCD;32.183;6288;1143.838;
6320;EThcD;32.292;6317;1144.171;Byonic:q-value=0.00104/GPS:MinICScore=8.665
6322;HCD;32.302;6317;1144.171;