TG004338

Peptide	R.EGVFVSN[H5N3F]GTHWFVTQR.N (PE001649)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N3F (N7)
Mod(s)
Molecular Weight	3,446.477
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.094	32.095	45.558	1,143.830	3+	1,532.675	2+	Y[pH4N2F]	66.8
32.094	32.095	45.558	1,143.830	3+	1,451.649	2+	Y[pH3N2F]	37.7
32.094	32.095	45.558	1,143.830	3+	1,459.646	2+	Y[pH4N2]	27.4
32.094	32.095	45.558	1,143.830	3+	1,378.620	2+	Y[pH3N2]	22.2
32.094	32.095	45.558	1,143.830	3+	1,034.001	2+	Y[pN]	18.2
32.094	32.095	45.558	1,143.830	3+	1,297.593	2+	Y[pH2N2]	15.0
32.094	32.095	45.558	1,143.830	3+	1,370.622	2+	Y[pH2N2F]	14.5
32.094	32.095	45.558	1,143.830	3+	1,107.030	2+	Y[pNF]	11.8
32.094	32.095	45.558	1,143.830	3+	1,613.701	2+	Y[pH5N2F]	19.5
32.094	32.095	45.558	1,143.830	3+	1,216.567	2+	Y[pHN2]	8.7
32.094	32.095	45.558	1,143.830	3+	1,135.540	2+	Y[pN2]	7.8
32.094	32.095	45.558	1,143.830	3+	1,289.596	2+	Y[pHN2F]	6.2
32.094	32.095	45.558	1,143.830	3+	1,208.569	2+	Y[pN2F]	5.4

Spectra

6132;EThcD;32.008;6127;1143.839;Byonic:q-value=0.000751/GPS:MinICScore=0.878
6134;HCD;32.018;6127;1143.839;
6164;EThcD;32.159;6162;1144.170;Byonic:q-value=0.000751/GPS:MinICScore=8.299
6166;HCD;32.170;6162;1144.170;