TG004331

Peptide	K.TPPIKDFGGFN[H3N5F]FSQILPDPSKPSK.R (PE001624)
Sequence	TPPIKDFGGFNFSQILPDPSKPSK
Glycans(s)	H3N5F (N11)
Mod(s)
Molecular Weight	4,281.983
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
45.892	45.668	79.155	1,067.001	4+	1,354.639	3+	Y[pH3N4F]	63.2
45.892	45.668	79.155	1,067.001	4+	1,300.621	3+	Y[pH2N4F]	22.5
45.892	45.668	79.155	1,067.001	4+	1,286.946	3+	Y[pH3N3F]	11.5
45.892	45.668	79.155	1,067.001	4+	1,232.928	3+	Y[pH2N3F]	8.9
45.892	45.668	79.155	1,067.001	4+	1,305.953	3+	Y[pH3N4]	8.8
45.892	45.668	79.155	1,067.001	4+	1,410.727	2+	Y[pN]	7.7
45.892	45.668	79.155	1,067.001	4+	1,848.888	2+	Y[pH2N3F]	5.7
45.892	45.668	79.155	1,067.001	4+	1,483.756	2+	Y[pNF]	5.3

Spectra

9193;EThcD;45.636;9186;1067.008;Byonic:q-value=2.23e-08/GPS:MinICScore=5.995
9195;HCD;45.645;9186;1067.008;
9289;EThcD;46.077;9283;1067.009;Byonic:q-value=6.44e-05/GPS:MinICScore=33.829
9291;HCD;46.139;9283;1067.009;