TG004329

Peptide	R.EGVFVSN[H3N4F]GTHWFVTQR.N (PE001512)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H3N4F (N7)
Mod(s)
Molecular Weight	3,325.451
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.432	32.543	46.492	1,103.488	3+	1,553.188	2+	Y[pH3N3F]	60.5
32.432	32.543	46.492	1,103.488	3+	1,472.162	2+	Y[pH2N3F]	40.7
32.432	32.543	46.492	1,103.488	3+	1,480.159	2+	Y[pH3N3]	14.5
32.432	32.543	46.492	1,103.488	3+	1,034.001	2+	Y[pN]	13.3
32.432	32.543	46.492	1,103.488	3+	1,297.593	2+	Y[pH2N2]	13.0
32.432	32.543	46.492	1,103.488	3+	1,399.133	2+	Y[pH2N3]	11.4
32.432	32.543	46.492	1,103.488	3+	1,216.567	2+	Y[pHN2]	7.2
32.432	32.543	46.492	1,103.488	3+	1,378.620	2+	Y[pH3N2]	6.6
32.432	32.543	46.492	1,103.488	3+	1,135.540	2+	Y[pN2]	5.9

Spectra

6347;EThcD;32.413;6340;1103.830;Byonic:q-value=0.00735/GPS:MinICScore=65.367
6349;HCD;32.432;6340;1103.830;