TG004316

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Peptide R.EGVFVSN[H4N5F]GTHWFVTQR.N (PE001528)
Sequence EGVFVSNGTHWFVTQR
Glycans(s) H4N5F (N7)
Mod(s)
Molecular Weight 3,690.583
Charge 3+
Protein S (P0DTC2)
Spectrum File MS_50_Covid_spike_trypsin_new
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction No enrichment
Sample SARS-CoV-2 Spike Protein

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
31.880 31.931 44.969 1,225.199 3+ 1,654.728 2+ Y[pH3N4F] 22.8
31.880 31.931 44.969 1,225.199 3+ 1,634.215 2+ Y[pH4N3F]* 22.2
31.880 31.931 44.969 1,225.199 3+ 1,735.754 2+ Y[pH4N4F] 19.5
31.880 31.931 44.969 1,225.199 3+ 1,480.159 2+ Y[pH3N3] 19.2
31.880 31.931 44.969 1,225.199 3+ 1,662.725 2+ Y[pH4N4] 12.7
31.880 31.931 44.969 1,225.199 3+ 1,553.188 2+ Y[pH3N3F] 12.1
31.880 31.931 44.969 1,225.199 3+ 1,581.699 2+ Y[pH3N4] 11.7
31.880 31.931 44.969 1,225.199 3+ 1,297.593 2+ Y[pH2N2] 10.4
31.880 31.931 44.969 1,225.199 3+ 1,034.001 2+ Y[pN] 9.7
31.880 31.931 44.969 1,225.199 3+ 1,378.620 2+ Y[pH3N2] 8.2
31.880 31.931 44.969 1,225.199 3+ 1,472.162 2+ Y[pH2N3F] 7.8
31.880 31.931 44.969 1,225.199 3+ 1,176.513 3+ Y[pH4N5] 6.3
31.880 31.931 44.969 1,225.199 3+ 1,399.133 2+ Y[pH2N3] 5.8
31.880 31.931 44.969 1,225.199 3+ 1,135.540 2+ Y[pN2] 5.0

Spectra

6231;EThcD;31.871;6226;1225.206;Byonic:q-value=0.00142/GPS:MinICScore=3.378
6233;HCD;31.880;6226;1225.206;