TG004284

Peptide	R.EGVFVSN[H5N4F]GTHWFVTQR.N (PE001533)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N4F (N7)
Mod(s)
Molecular Weight	3,649.556
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
31.830	31.850	44.767	1,211.523	3+	1,634.215	2+	Y[pH4N3F]	60.2
31.830	31.850	44.767	1,211.523	3+	1,553.188	2+	Y[pH3N3F]	27.0
31.830	31.850	44.767	1,211.523	3+	1,480.159	2+	Y[pH3N3]	22.6
31.830	31.850	44.767	1,211.523	3+	1,297.593	2+	Y[pH2N2]	11.5
31.830	31.850	44.767	1,211.523	3+	1,034.001	2+	Y[pN]	11.6
31.830	31.850	44.767	1,211.523	3+	1,378.620	2+	Y[pH3N2]	11.1
31.830	31.850	44.767	1,211.523	3+	1,451.649	2+	Y[pH3N2F]*	7.2
31.830	31.850	44.767	1,211.523	3+	1,370.622	2+	Y[pH2N2F]*	7.0
31.830	31.850	44.767	1,211.523	3+	1,135.540	2+	Y[pN2]	5.4
31.830	31.850	44.767	1,211.523	3+	1,216.567	2+	Y[pHN2]	5.4

Spectra

6211;EThcD;31.782;6208;1211.867;Byonic:q-value=0.00346/GPS:MinICScore=97.956
6213;HCD;31.791;6208;1211.867;
6220;EThcD;31.821;6219;1212.201;Byonic:q-value=0.00104/GPS:MinICScore=23.817
6222;HCD;31.830;6219;1212.201;
6254;EThcD;31.981;6249;1211.532;Byonic:q-value=0.00104/GPS:MinICScore=8.496
6255;HCD;31.987;6249;1211.532;