TG004225

Peptide	R.EGVFVSN[H6N5FS2]GTHWFVTQR.N (PE001613)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N5FS2 (N7)
Mod(s)
Molecular Weight	4,596.879
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
43.023	42.869	72.189	1,145.725	4+	1,634.215	2+	Y[pH4N3F]	11.8
43.023	42.869	72.189	1,145.725	4+	1,553.188	2+	Y[pH3N3F]	11.1
43.023	42.869	72.189	1,145.725	4+	1,370.622	2+	Y[pH2N2F]*	9.7
43.023	42.869	72.189	1,145.725	4+	1,816.781	2+	Y[pH5N4F]	7.2
43.023	42.869	72.189	1,145.725	4+	1,107.030	2+	Y[pNF]*	6.0
43.023	42.869	72.189	1,145.725	4+	1,034.001	2+	Y[pN]	5.7
43.023	42.869	72.189	1,145.725	4+	1,297.593	2+	Y[pH2N2]	5.5
43.023	42.869	72.189	1,145.725	4+	1,735.754	2+	Y[pH4N4F]	5.4
43.023	42.869	72.189	1,145.725	4+	1,480.159	2+	Y[pH3N3]	5.3
43.023	42.869	72.189	1,145.725	4+	1,308.555	3+	Y[pH5N4FS]	5.0

Spectra

8637;EThcD;43.014;8632;1145.735;Byonic:q-value=0.00104/GPS:MinICScore=29.411
8639;HCD;43.023;8632;1145.735;