TG004214

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Peptide R.EGVFVSN[H5N3]GTHWFVTQR.N (PE001515)
Sequence EGVFVSNGTHWFVTQR
Glycans(s) H5N3 (N7)
Mod(s)
Molecular Weight 3,300.419
Charge 3+
Protein S (P0DTC2)
Spectrum File MS_50_Covid_spike_trypsin_new
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction No enrichment
Sample SARS-CoV-2 Spike Protein

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
32.489 32.526 46.449 1,095.144 3+ 1,459.646 2+ Y[pH4N2] 83.4
32.489 32.526 46.449 1,095.144 3+ 1,378.620 2+ Y[pH3N2] 43.7
32.489 32.526 46.449 1,095.144 3+ 1,540.672 2+ Y[pH5N2]* 42.5
32.489 32.526 46.449 1,095.144 3+ 1,034.001 2+ Y[pN] 29.6
32.489 32.526 46.449 1,095.144 3+ 1,297.593 2+ Y[pH2N2] 21.2
32.489 32.526 46.449 1,095.144 3+ 1,216.567 2+ Y[pHN2] 13.9
32.489 32.526 46.449 1,095.144 3+ 1,135.540 2+ Y[pN2] 12.1

Spectra

6346;EThcD;32.408;6340;1095.485;Byonic:q-value=0.00399/GPS:MinICScore=71.902
6348;HCD;32.427;6340;1095.485;
6374;EThcD;32.542;6369;1095.151;Byonic:q-value=0.00104/GPS:MinICScore=7.527
6376;HCD;32.551;6369;1095.151;