TG004212

Peptide	R.DLPQGFSALEPLVDLPIGIN[H5N3S]ITR.F (PE001497)
Sequence	DLPQGFSALEPLVDLPIGINITR
Glycans(s)	H5N3S (N20)
Mod(s)
Molecular Weight	4,205.961
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
81.639	81.785	169.038	1,396.991	3+	1,341.224	2+	Y[pN]	51.7
81.639	81.785	169.038	1,396.991	3+	1,685.843	2+	Y[pH3N2]	39.6
81.639	81.785	169.038	1,396.991	3+	1,766.869	2+	Y[pH4N2]	38.6
81.639	81.785	169.038	1,396.991	3+	1,604.817	2+	Y[pH2N2]	20.3
81.639	81.785	169.038	1,396.991	3+	1,442.764	2+	Y[pN2]	11.6
81.639	81.785	169.038	1,396.991	3+	1,523.790	2+	Y[pHN2]	11.2
81.639	81.785	169.038	1,396.991	3+	1,868.409	2+	Y[pH4N3]	5.3

Spectra

16461;EThcD;81.092;16460;1397.004;Byonic:q-value=1.75e-05/GPS:MinICScore=28.259
16462;HCD;81.106;16460;1397.004;
16533;EThcD;81.460;16532;1397.002;Byonic:q-value=7.59e-06/GPS:MinICScore=19.963
16535;HCD;81.470;16532;1397.002;
16600;EThcD;81.800;16598;1397.001;Byonic:q-value=3.82e-07/GPS:MinICScore=2.794
16601;HCD;81.807;16598;1397.001;
16662;EThcD;82.149;16661;1397.001;Byonic:q-value=1.23e-05/GPS:MinICScore=4.293
16663;HCD;82.157;16661;1397.001;