TG004192

Peptide	R.EGVFVSN[H3N3F]GTHWFVTQR.N (PE001611)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H3N3F (N7)
Mod(s)
Molecular Weight	3,122.371
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.770	32.817	47.173	1,035.795	3+	1,370.622	2+	Y[pH2N2F]	38.7
32.770	32.817	47.173	1,035.795	3+	1,451.649	2+	Y[pH3N2F]	33.3
32.770	32.817	47.173	1,035.795	3+	1,297.593	2+	Y[pH2N2]	17.6
32.770	32.817	47.173	1,035.795	3+	1,034.001	2+	Y[pN]	12.8
32.770	32.817	47.173	1,035.795	3+	1,378.620	2+	Y[pH3N2]	9.8
32.770	32.817	47.173	1,035.795	3+	1,289.596	2+	Y[pHN2F]	8.5
32.770	32.817	47.173	1,035.795	3+	1,216.567	2+	Y[pHN2]	7.7
32.770	32.817	47.173	1,035.795	3+	1,107.030	2+	Y[pNF]	7.7
32.770	32.817	47.173	1,035.795	3+	1,135.540	2+	Y[pN2]	6.1

Spectra

6420;EThcD;32.761;6413;1035.800;Byonic:q-value=0.00735/GPS:MinICScore=55.906
6422;HCD;32.770;6413;1035.800;