TG004185

Peptide	R.EGVFVSN[H6N3]GTHWFVTQR.N (PE001513)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N3 (N7)
Mod(s)
Molecular Weight	3,462.472
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.163	32.094	45.555	1,149.162	3+	1,540.672	2+	Y[pH5N2]	85.7
32.163	32.094	45.555	1,149.162	3+	1,459.646	2+	Y[pH4N2]	58.4
32.163	32.094	45.555	1,149.162	3+	1,034.001	2+	Y[pN]	27.0
32.163	32.094	45.555	1,149.162	3+	1,378.620	2+	Y[pH3N2]	25.0
32.163	32.094	45.555	1,149.162	3+	1,297.593	2+	Y[pH2N2]	16.5
32.163	32.094	45.555	1,149.162	3+	1,621.699	2+	Y[pH6N2]*	13.7
32.163	32.094	45.555	1,149.162	3+	1,216.567	2+	Y[pHN2]	10.0
32.163	32.094	45.555	1,149.162	3+	1,135.540	2+	Y[pN2]	9.8

Spectra

6103;EThcD;31.865;6095;1149.505;Byonic:q-value=0.000751/GPS:ICScore=272.748
6105;HCD;31.875;6095;1149.505;
6223;EThcD;32.441;6214;1149.168;Byonic:q-value=0.00184/GPS:ICScore=10.619
6225;HCD;32.451;6214;1149.168;