TG004157

Peptide	K.DFGGFN[H5N4S]FSQILPDPSKPSKR.S (PE001488)
Sequence	DFGGFNFSQILPDPSKPSKR
Glycans(s)	H5N4S (N6)
Mod(s)
Molecular Weight	4,167.815
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
50.850	50.881	92.810	1,038.459	4+	1,287.244	3+	Y[pH5N4]	51.8
50.850	50.881	92.810	1,038.459	4+	1,165.534	3+	Y[pH4N3]	30.0
50.850	50.881	92.810	1,038.459	4+	1,233.227	3+	Y[pH4N4]	28.8
50.850	50.881	92.810	1,038.459	4+	1,666.770	2+	Y[pH3N3]	24.8
50.850	50.881	92.810	1,038.459	4+	1,747.796	2+	Y[pH4N3]	22.9
50.850	50.881	92.810	1,038.459	4+	1,484.204	2+	Y[pH2N2]	20.3
50.850	50.881	92.810	1,038.459	4+	1,565.230	2+	Y[pH3N2]	19.2
50.850	50.881	92.810	1,038.459	4+	1,220.611	2+	Y[pN]	18.2
50.850	50.881	92.810	1,038.459	4+	1,111.516	3+	Y[pH3N3]	10.9
50.850	50.881	92.810	1,038.459	4+	1,403.177	2+	Y[pHN2]	7.8
50.850	50.881	92.810	1,038.459	4+	1,585.744	2+	Y[pH2N3]	6.5
50.850	50.881	92.810	1,038.459	4+	1,322.151	2+	Y[pN2]	5.8

Spectra

10280;EThcD;50.840;10272;1038.466;Byonic:q-value=0.00184/GPS:MinICScore=26.682
10282;HCD;50.850;10272;1038.466;