TG004130

Peptide	R.EGVFVSN[H3N4F]GTHWFVTQR.N (PE001512)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H3N4F (N7)
Mod(s)
Molecular Weight	3,325.451
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.266	32.372	46.255	1,103.488	3+	1,553.188	2+	Y[pH3N3F]	54.0
32.266	32.372	46.255	1,103.488	3+	1,472.162	2+	Y[pH2N3F]	35.6
32.266	32.372	46.255	1,103.488	3+	1,370.622	2+	Y[pH2N2F]*	17.6
32.266	32.372	46.255	1,103.488	3+	1,480.159	2+	Y[pH3N3]	14.3
32.266	32.372	46.255	1,103.488	3+	1,297.593	2+	Y[pH2N2]	13.4
32.266	32.372	46.255	1,103.488	3+	1,034.001	2+	Y[pN]	12.2
32.266	32.372	46.255	1,103.488	3+	1,399.133	2+	Y[pH2N3]	11.3
32.266	32.372	46.255	1,103.488	3+	1,451.649	2+	Y[pH3N2F]*	7.4
32.266	32.372	46.255	1,103.488	3+	1,216.567	2+	Y[pHN2]	7.3
32.266	32.372	46.255	1,103.488	3+	1,107.030	2+	Y[pNF]*	7.1
32.266	32.372	46.255	1,103.488	3+	1,378.620	2+	Y[pH3N2]	6.0
32.266	32.372	46.255	1,103.488	3+	1,135.540	2+	Y[pN2]	5.4
32.266	32.372	46.255	1,103.488	3+	1,289.596	2+	Y[pHN2F]*	5.4

Spectra

6180;EThcD;32.236;6174;1103.493;Byonic:q-value=0.00475/GPS:MinICScore=281.407
6182;HCD;32.245;6174;1103.493;
6189;EThcD;32.278;6186;1103.830;Byonic:q-value=0.000136/GPS:MinICScore=42.966
6191;HCD;32.288;6186;1103.830;