TG004127

Peptide	K.TPPIKDFGGFN[H5N4FS2]FSQILPDPSKPSK.R (PE001564)
Sequence	TPPIKDFGGFNFSQILPDPSKPSK
Glycans(s)	H5N4FS2 (N11)
Mod(s)
Molecular Weight	4,985.2
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
56.196	56.126	106.003	1,242.805	4+	1,340.963	3+	Y[pH4N3F]	52.6
56.196	56.126	106.003	1,242.805	4+	1,170.566	3+	Y[pH3N2]	31.6
56.196	56.126	106.003	1,242.805	4+	1,437.995	3+	Y[pH4N3FS]	29.4
56.196	56.126	106.003	1,242.805	4+	1,462.674	3+	Y[pH5N4F]	26.9
56.196	56.126	106.003	1,242.805	4+	1,286.946	3+	Y[pH3N3F]	26.7
56.196	56.126	106.003	1,242.805	4+	1,408.656	3+	Y[pH4N4F]	21.3
56.196	56.126	106.003	1,242.805	4+	1,559.706	3+	Y[pH5N4FS]	13.0
56.196	56.126	106.003	1,242.805	4+	1,097.257	4+	Y[pH5N4F]	9.7
56.196	56.126	106.003	1,242.805	4+	1,747.348	2+	Y[pH2N2F]*	9.2
56.196	56.126	106.003	1,242.805	4+	1,292.277	3+	Y[pH4N3]	8.7
56.196	56.126	106.003	1,242.805	4+	1,238.260	3+	Y[pH3N3]	7.9
56.196	56.126	106.003	1,242.805	4+	1,505.688	3+	Y[pH4N4FS]	7.4
56.196	56.126	106.003	1,242.805	4+	1,929.914	2+	Y[pH3N3F]	7.4
56.196	56.126	106.003	1,242.805	4+	1,483.756	2+	Y[pNF]*	7.3
56.196	56.126	106.003	1,242.805	4+	1,383.977	3+	Y[pH3N3FS]	7.2
56.196	56.126	106.003	1,242.805	4+	1,410.727	2+	Y[pN]	6.9
56.196	56.126	106.003	1,242.805	4+	1,828.375	2+	Y[pH3N2F]*	6.9
56.196	56.126	106.003	1,242.805	4+	1,674.319	2+	Y[pH2N2]	5.9

Spectra

11451;EThcD;56.181;11441;1243.063;Byonic:q-value=0.000751/GPS:MinICScore=82.750
11453;HCD;56.196;11441;1243.063;