TG000411

Peptide	K.LSYTCEGGFRISEEN[H5N4S2]ETTCYMGK.W (PE000178)
Sequence	LSYTCEGGFRISEENETTCYMGK
Glycans(s)	H5N4S2 (N15)
Mod(s)	57.021 (C5), 57.021 (C19)
Molecular Weight	4,953.939
Charge	4+
Protein	CFH (P08603)
Spectrum File	MS_24_UO1_CFH_UO1
Method	Orbitrap Fusion-Lumos, DDA (HCD pd EThcD), nano LC-MS/MS
Analytical Fraction	CFH Protein Standard
Sample	Human Serum

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
42.660	42.649		1,234.996	4+	1,468.138	2+	Y[pN]	40.4
42.660	42.649		1,234.996	4+	1,812.757	2+	Y[pH3N2]	36.4
42.660	42.649		1,234.996	4+	1,731.731	2+	Y[pH2N2]	30.9
42.660	42.649		1,234.996	4+	1,549.294	3+	Y[pH5N4S]	22.6
42.660	42.649		1,234.996	4+	1,330.551	3+	Y[pH4N3]	18.4
42.660	42.649		1,234.996	4+	1,995.323	2+	Y[pH4N3]	17.4
42.660	42.649		1,234.996	4+	1,452.262	3+	Y[pH5N4]	15.0
42.660	42.649		1,234.996	4+	1,276.534	3+	Y[pH3N3]	12.7
42.660	42.649		1,234.996	4+	1,914.297	2+	Y[pH3N3]	12.3
42.660	42.649		1,234.996	4+	1,373.566	3+	Y[pH3N3S]	10.2
42.660	42.649		1,234.996	4+	1,650.704	2+	Y[pHN2]	8.4
42.660	42.649		1,234.996	4+	1,569.678	2+	Y[pN2]	7.8
42.660	42.649		1,234.996	4+	979.095	3+	Y[pN]	6.9
42.660	42.649		1,234.996	4+	1,495.276	3+	Y[pH4N4S]	6.6

Spectra

10838;HCD;42.520;10836;1234.985;Byonic:q-value=6.39e-06/GPS:MinICScore=114.237
10840;EThcD;42.524;10836;1234.985;
10943;HCD;42.799;10942;1235.236;Byonic:q-value=2.58e-05/GPS:MinICScore=66.579
10945;EThcD;42.806;10942;1235.236;