TG000394

Peptide	R.ISEEN[H5N4S2]ETTCYM[Ox]GK.W (PE000177)
Sequence	ISEENETTCYMGK
Glycans(s)	H5N4S2 (N5)
Mod(s)	57.021 (C9), 15.995 (M11)
Molecular Weight	3,799.422
Charge	3+
Protein	CFH (P08603)
Spectrum File	MS_24_UO1_CFH_UO1
Method	Orbitrap Fusion-Lumos, DDA (HCD pd EThcD), nano LC-MS/MS
Analytical Fraction	CFH Protein Standard
Sample	Human Serum

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
20.336	25.092		1,261.482	3+	1,563.606	2+	Y[pH4N3S]	31.3
20.336	25.092		1,261.482	3+	1,418.058	2+	Y[pH4N3]	27.2
20.336	25.092		1,261.482	3+	1,780.738	1+	Y[pN]	16.7
20.336	25.092		1,261.482	3+	1,337.032	2+	Y[pH3N3]	12.3
20.336	25.092		1,261.482	3+	1,482.579	2+	Y[pH3N3S]	12.0
20.336	25.092		1,261.482	3+	890.873	2+	Y[pN]	11.7
20.336	25.092		1,261.482	3+	1,746.172	2+	Y[pH5N4S]	8.3
20.336	25.092		1,261.482	3+	1,600.624	2+	Y[pH5N4]	6.9
20.336	25.092		1,261.482	3+	1,154.466	2+	Y[pH2N2]	6.4

Spectra

3664;HCD;18.897;3662;1261.473;Byonic:q-value=5.18e-06/GPS:MinICScore=11.060
3666;EThcD;18.901;3662;1261.473;
3755;HCD;19.288;3752;1261.472;Byonic:q-value=3.84e-06/GPS:MinICScore=43.951
3757;EThcD;19.297;3752;1261.472;
4306;HCD;21.384;4296;1261.808;Byonic:q-value=1e-05/GPS:MinICScore=85.559
4308;EThcD;21.388;4296;1261.808;
5170;HCD;24.957;5164;1261.806;Byonic:q-value=0.00141/GPS:MinICScore=86.008
5172;EThcD;24.966;5164;1261.806;