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Suspension HEK293F cells (Invitrogen) grown in serum-free 293 Freestyle medium (Invitrogen).
Proteins
|
|
Gene
|
Description
|
Species
|
Peptides
|
| P10909
|
CLU
|
Clusterin
|
Homo sapiens
|
2 / 28
|
| P01861
|
IGHG4
|
Immunoglobulin heavy constant gamma 4
|
Homo sapiens
|
8 / 24
|
| P02790
|
HPX
|
Hemopexin
|
Homo sapiens
|
1 / 19
|
| P16870
|
CPE
|
Carboxypeptidase E
|
Homo sapiens
|
14 / 14
|
| O75882
|
ATRN
|
Attractin
|
Homo sapiens
|
1 / 9
|
| Q08380
|
LGALS3BP
|
Galectin-3-binding protein
|
Homo sapiens
|
9 / 9
|
| P13473
|
LAMP2
|
Lysosome-associated membrane glycoprotein 2
|
Homo sapiens
|
9 / 9
|
| Q15293
|
RCN1
|
Reticulocalbin-1
|
Homo sapiens
|
7 / 7
|
| Q9H3G5
|
CPVL
|
Probable serine carboxypeptidase CPVL
|
Homo sapiens
|
6 / 6
|
| P11279
|
LAMP1
|
Lysosome-associated membrane glycoprotein 1
|
Homo sapiens
|
6 / 6
|
| P36955
|
SERPINF1
|
Pigment epithelium-derived factor
|
Homo sapiens
|
2 / 5
|
| P01033
|
TIMP1
|
Metalloproteinase inhibitor 1
|
Homo sapiens
|
5 / 5
|
| Q92542
|
NCSTN
|
Nicastrin
|
Homo sapiens
|
5 / 5
|
| P14625
|
HSP90B1
|
Endoplasmin
|
Homo sapiens
|
5 / 5
|
| Q9Y4L1
|
HYOU1
|
Hypoxia up-regulated protein 1
|
Homo sapiens
|
4 / 4
|
| Q9UHG3
|
PCYOX1
|
Prenylcysteine oxidase 1
|
Homo sapiens
|
4 / 4
|
| Q92820
|
GGH
|
Gamma-glutamyl hydrolase
|
Homo sapiens
|
4 / 4
|
| Q96AY3
|
FKBP10
|
Peptidyl-prolyl cis-trans isomerase FKBP10
|
Homo sapiens
|
3 / 3
|
| Q92626
|
PXDN
|
Peroxidasin homolog
|
Homo sapiens
|
3 / 3
|
| Q9HDC9
|
APMAP
|
Adipocyte plasma membrane-associated protein
|
Homo sapiens
|
2 / 2
|
... further results
Glycans
|
|
Name
|
Mol. Wt.
|
Classification
|
Peptides
|
| G00912UN
|
H5N4S2
|
2,222.783
|
N-Glycan
|
3 / 227
|
| G59626AS
|
H5N4S
|
1,931.688
|
N-Glycan
|
2 / 126
|
| G88374WZ
|
H5N4FS2
|
2,368.841
|
N-Glycan
|
4 / 82
|
| G08293MJ
|
H5N4FS
|
2,077.745
|
N-Glycan
|
4 / 71
|
| G83555HU
|
H5N4F
|
1,786.65
|
N-Glycan
|
2 / 40
|
| G25987BV
|
H3N4F
|
1,462.544
|
N-Glycan
|
2 / 33
|
| G02815KT
|
H5N2
|
1,234.433
|
N-Glycan
|
15 / 30
|
| G59937CP
|
H4N4F
|
1,624.597
|
N-Glycan
|
2 / 30
|
| G41247ZX
|
H6N2
|
1,396.486
|
N-Glycan
|
20 / 30
|
| G85144OK
|
H6N5FS2
|
2,733.973
|
N-Glycan
|
2 / 28
|
| G62765YT
|
H8N2
|
1,720.592
|
N-Glycan
|
17 / 22
|
| G68318VE
|
H4N5F
|
1,827.677
|
N-Glycan
|
2 / 21
|
| G88725PI
|
H3N5F
|
1,665.624
|
N-Glycan
|
2 / 19
|
| G80920RR
|
H9N2
|
1,882.645
|
N-Glycan
|
16 / 19
|
| G40926MX
|
H6N5S
|
2,296.82
|
N-Glycan
|
1 / 19
|
| G12580WI
|
H5N5FS
|
2,280.825
|
N-Glycan
|
3 / 19
|
| G80475RE
|
H3N3F
|
1,259.465
|
N-Glycan
|
2 / 16
|
| G45504EY
|
H4N4S
|
1,769.635
|
N-Glycan
|
1 / 14
|
| G95678HJ
|
H7N6FS4
|
3,681.296
|
N-Glycan
|
3 / 14
|
| G44953PJ
|
H4N5FS
|
2,118.772
|
N-Glycan
|
2 / 13
|
... further results
Acquisitions
| Spectrum File | Anal. Fraction | Method | Trans. Groups |
|---|
| MS_47_UO1_HEK_1_1n | SAX-ERLICH Enrichment | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 4 |
| MS_47_UO1_HEK_1_2n | SAX-ERLICH Enrichment | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 9 |
| MS_47_UO1_HEK_2_1n | SAX-ERLICH Enrichment | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 17 |
| MS_47_UO1_HEK_2_2n | SAX-ERLICH Enrichment | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 1 |
| MS_51_CM_20210210173056 | No enrichment | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 8 |
| MS_51_CM2 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 4 |
| MS_51_CM3 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 11 |
| MS_51_HEK_01_EThcDpdHCD_20210204023608 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 16 |
| MS_51_HEK_CM02 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 9 |
| MS_51_HEK_acetone | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 30 |
| MS_51_HEK_acetone_z2incl | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 41 |
| MS_51_HEK_acetone2 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 27 |
| MS_51_HEK02_20210214173011 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 4 |
| MS_52_HEK_secretome01 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 15 |
| MS_52_HEK_secretome02 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 6 |
| MS_52_HEK_secretome03 | | Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS | 14 |