TG006611

Peptide	R.HN[H6N5FS2]STGCLR.M (PE002202)
Sequence	HNSTGCLR
Glycans(s)	H6N5FS2 (N2)
Mod(s)	57.021 (C6)
Molecular Weight	3,677.403
Charge	3+
Protein	CLU (P10909)
Spectrum File	MS_52_HEK_secretome03
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction
Sample	HEK293 Cells

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
12.433	12.475	4.667	1,220.809	3+	1,502.596	2+	Y[pH5N4FS]	100.0
12.433	12.475	4.667	1,220.809	3+	1,357.049	2+	Y[pH5N4F]	63.0
12.433	12.475	4.667	1,220.809	3+	1,648.144	2+	Y[pH5N4FS2]*	19.4
12.433	12.475	4.667	1,220.809	3+	1,174.482	2+	Y[pH4N3F]	15.8
12.433	12.475	4.667	1,220.809	3+	1,276.022	2+	Y[pH4N4F]	15.2
12.433	12.475	4.667	1,220.809	3+	1,685.162	2+	Y[pH6N5FS]	11.8
12.433	12.475	4.667	1,220.809	3+	1,093.456	2+	Y[pH3N3F]	10.8
12.433	12.475	4.667	1,220.809	3+	1,421.570	2+	Y[pH4N4FS]	10.3
12.433	12.475	4.667	1,220.809	3+	1,284.020	2+	Y[pH5N4]	9.9
12.433	12.475	4.667	1,220.809	3+	1,293.587	1+	Y[pNF]*	9.4
12.433	12.475	4.667	1,220.809	3+	1,539.615	2+	Y[pH6N5F]	8.0
12.433	12.475	4.667	1,220.809	3+	1,101.454	2+	Y[pH4N3]	7.4
12.433	12.475	4.667	1,220.809	3+	1,458.588	2+	Y[pH5N5F]	5.6

Spectra

2152;EThcD;12.407;2150;1221.135;Byonic:q-value=0.00117/GPS:MinICScore=146.420
2154;HCD;12.433;2150;1221.135;