TG006586

Peptide	K.GN[H6N3F]ETIVNLIHSTR.I (PE002163)
Sequence	GNETIVNLIHSTR
Glycans(s)	H6N3F (N2)
Mod(s)
Molecular Weight	3,198.392
Charge	3+
Protein	CPE (P16870)
Spectrum File	MS_52_HEK_secretome01
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction
Sample	HEK293 Cells

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
18.483	18.448	24.673	1,061.135	3+	1,335.603	2+	Y[pH5N2]	100.0
18.483	18.448	24.673	1,061.135	3+	1,254.577	2+	Y[pH4N2]	69.8
18.483	18.448	24.673	1,061.135	3+	1,408.632	2+	Y[pH5N2F]*	62.0
18.483	18.448	24.673	1,061.135	3+	1,173.551	2+	Y[pH3N2]	32.0
18.483	18.448	24.673	1,061.135	3+	1,327.606	2+	Y[pH4N2F]*	27.3
18.483	18.448	24.673	1,061.135	3+	1,092.524	2+	Y[pH2N2]	20.7
18.483	18.448	24.673	1,061.135	3+	1,656.856	1+	Y[pN]	17.4
18.483	18.448	24.673	1,061.135	3+	828.932	2+	Y[pN]	15.9
18.483	18.448	24.673	1,061.135	3+	930.471	2+	Y[pN2]	15.0
18.483	18.448	24.673	1,061.135	3+	1,011.498	2+	Y[pHN2]	12.1
18.483	18.448	24.673	1,061.135	3+	1,246.580	2+	Y[pH3N2F]*	6.0
18.483	18.448	24.673	1,061.135	3+	885.407	3+	Y[pH4N2F]*	11.5

Spectra

3684;EThcD;18.368;3673;1061.468;Byonic:q-value=3.27e-06/GPS:MinICScore=22.091
3685;HCD;18.375;3673;1061.468;
3732;EThcD;18.584;3725;1061.132;Byonic:q-value=0.000482/GPS:MinICScore=25.315
3734;HCD;18.592;3725;1061.132;