TG006400

Peptide	R.EGVFVSN[H6N3F]GTHWFVTQR.N (PE002182)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N3F (N7)
Mod(s)
Molecular Weight	3,608.53
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
31.977	31.957	45.033	1,197.848	3+	1,613.701	2+	Y[pH5N2F]*	65.4
31.977	31.957	45.033	1,197.848	3+	1,532.675	2+	Y[pH4N2F]*	42.1
31.977	31.957	45.033	1,197.848	3+	1,540.672	2+	Y[pH5N2]	40.0
31.977	31.957	45.033	1,197.848	3+	1,459.646	2+	Y[pH4N2]	30.8
31.977	31.957	45.033	1,197.848	3+	1,378.620	2+	Y[pH3N2]	15.1
31.977	31.957	45.033	1,197.848	3+	1,034.001	2+	Y[pN]	14.6
31.977	31.957	45.033	1,197.848	3+	1,451.649	2+	Y[pH3N2F]*	13.5
31.977	31.957	45.033	1,197.848	3+	1,297.593	2+	Y[pH2N2]	8.9
31.977	31.957	45.033	1,197.848	3+	1,370.622	2+	Y[pH2N2F]*	7.6
31.977	31.957	45.033	1,197.848	3+	1,107.030	2+	Y[pNF]*	6.6
31.977	31.957	45.033	1,197.848	3+	1,135.540	2+	Y[pN2]	6.6
31.977	31.957	45.033	1,197.848	3+	1,143.830	3+	Y[pH5N3F]	5.5
31.977	31.957	45.033	1,197.848	3+	1,216.567	2+	Y[pHN2]	5.4

Spectra

6243;EThcD;31.932;6238;1198.190;Byonic:q-value=0.00104/GPS:MinICScore=2.533
6245;HCD;31.941;6238;1198.190;
6259;EThcD;32.003;6256;1197.857;Byonic:q-value=0.000175/GPS:MinICScore=41.264
6261;HCD;32.013;6256;1197.857;