TG006399

Peptide	R.EGVFVSN[H6N7]GTHWFVTQR.N (PE001650)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N7 (N7)
Mod(s)
Molecular Weight	4,274.789
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
31.140	31.227	43.217	1,065.203	4+	1,764.265	2+	Y[pH4N5]	20.5
31.140	31.227	43.217	1,065.203	4+	1,845.292	2+	Y[pH5N5]	7.3
31.140	31.227	43.217	1,065.203	4+	1,581.699	2+	Y[pH3N4]	7.6
31.140	31.227	43.217	1,065.203	4+	1,034.001	2+	Y[pN]	6.3
31.140	31.227	43.217	1,065.203	4+	1,662.725	2+	Y[pH4N4]	6.1
31.140	31.227	43.217	1,065.203	4+	1,500.673	2+	Y[pH2N4]	6.0
31.140	31.227	43.217	1,065.203	4+	1,683.239	2+	Y[pH3N5]	5.0

Spectra

6035;EThcD;30.956;6033;1065.713;Byonic:q-value=0.00346/GPS:MinICScore=152.081
6037;HCD;30.965;6033;1065.713;
6072;EThcD;31.132;6066;1065.460;Byonic:q-value=0.00346/GPS:MinICScore=36.936
6074;HCD;31.140;6066;1065.460;
6091;EThcD;31.212;6090;1065.209;Byonic:q-value=0.000245/GPS:MinICScore=81.654
6093;HCD;31.221;6090;1065.209;