TG004830

Peptide	R.ISFNFFVTAPKN[H5N4FS]VSDIIPR.T (PE001688)
Sequence	ISFNFFVTAPKNVSDIIPR
Glycans(s)	H5N4FS (N12)
Mod(s)
Molecular Weight	4,241.914
Charge	4+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
57.026	57.001	108.194	1,056.984	4+	1,602.279	2+	Y[pH3N2F]*	31.6
57.026	57.001	108.194	1,056.984	4+	1,521.253	2+	Y[pH2N2F]*	26.0
57.026	57.001	108.194	1,056.984	4+	1,784.846	2+	Y[pH4N3F]	18.9
57.026	57.001	108.194	1,056.984	4+	1,703.819	2+	Y[pH3N3F]	18.3
57.026	57.001	108.194	1,056.984	4+	1,448.224	2+	Y[pH2N2]	13.9
57.026	57.001	108.194	1,056.984	4+	1,257.661	2+	Y[pNF]*	13.4
57.026	57.001	108.194	1,056.984	4+	1,184.632	2+	Y[pN]	12.8
57.026	57.001	108.194	1,056.984	4+	1,529.250	2+	Y[pH3N2]	11.1
57.026	57.001	108.194	1,056.984	4+	1,440.227	2+	Y[pHN2F]*	8.6
57.026	57.001	108.194	1,056.984	4+	1,630.790	2+	Y[pH3N3]	6.9
57.026	57.001	108.194	1,056.984	4+	1,190.233	3+	Y[pH4N3F]	6.8
57.026	57.001	108.194	1,056.984	4+	1,622.793	2+	Y[pH2N3F]	5.0
57.026	57.001	108.194	1,056.984	4+	1,311.944	3+	Y[pH5N4F]	5.1

Spectra

11253;EThcD;56.727;11247;1056.990;Byonic:q-value=3.13e-06/GPS:MinICScore=34.629
11255;HCD;56.735;11247;1056.990;
11347;EThcD;57.162;11335;1056.992;Byonic:q-value=1.1e-07/GPS:MinICScore=85.164
11348;HCD;57.168;11335;1056.992;
11354;EThcD;57.191;11349;1057.238;Byonic:q-value=1.15e-07/GPS:MinICScore=15.708
11356;HCD;57.200;11349;1057.238;