TG004823

Peptide	K.LQLQALQQN[H5N4FS2]GSSVLSEDKSK.R (PE001731)
Sequence	LQLQALQQNGSSVLSEDKSK
Glycans(s)	H5N4FS2 (N9)
Mod(s)
Molecular Weight	4,540.98
Charge	4+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
35.354	34.914	52.721	1,131.750	4+	1,188.617	2+	Y[pN]	15.2
35.354	34.914	52.721	1,131.750	4+	1,525.238	2+	Y[pH2N2F]*	14.7
35.354	34.914	52.721	1,131.750	4+	1,606.265	2+	Y[pH3N2F]*	14.4
35.354	34.914	52.721	1,131.750	4+	1,452.209	2+	Y[pH2N2]	13.4
35.354	34.914	52.721	1,131.750	4+	1,707.804	2+	Y[pH3N3F]	13.0
35.354	34.914	52.721	1,131.750	4+	1,289.922	3+	Y[pH4N3FS]	11.3
35.354	34.914	52.721	1,131.750	4+	1,788.831	2+	Y[pH4N3F]	11.0
35.354	34.914	52.721	1,131.750	4+	1,411.632	3+	Y[pH5N4FS]	10.6
35.354	34.914	52.721	1,131.750	4+	1,261.646	2+	Y[pNF]*	10.5
35.354	34.914	52.721	1,131.750	4+	1,533.236	2+	Y[pH3N2]	10.4
35.354	34.914	52.721	1,131.750	4+	1,192.890	3+	Y[pH4N3F]	10.2
35.354	34.914	52.721	1,131.750	4+	1,314.601	3+	Y[pH5N4F]	9.2
35.354	34.914	52.721	1,131.750	4+	1,634.775	2+	Y[pH3N3]	8.3
35.354	34.914	52.721	1,131.750	4+	1,715.802	2+	Y[pH4N3]	6.7
35.354	34.914	52.721	1,131.750	4+	1,371.183	2+	Y[pHN2]	5.5

Spectra

6470;EThcD;35.348;6466;1131.756;Byonic:q-value=5.09e-06/GPS:MinICScore=15.104
6471;HCD;35.354;6466;1131.756;