TG004807

Peptide	K.LQLQALQQN[H5N4FS]GSSVLSEDKSKR.L (PE001786)
Sequence	LQLQALQQNGSSVLSEDKSKR
Glycans(s)	H5N4FS (N9)
Mod(s)
Molecular Weight	4,405.985
Charge	4+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
24.465	24.823	27.377	1,098.001	4+	1,244.924	3+	Y[pH4N3F]	100.0
24.465	24.823	27.377	1,098.001	4+	1,366.634	3+	Y[pH5N4F]	80.7
24.465	24.823	27.377	1,098.001	4+	1,190.906	3+	Y[pH3N3F]	23.7
24.465	24.823	27.377	1,098.001	4+	1,684.315	2+	Y[pH3N2F]*	16.2
24.465	24.823	27.377	1,098.001	4+	1,603.289	2+	Y[pH2N2F]*	15.4
24.465	24.823	27.377	1,098.001	4+	1,312.617	3+	Y[pH4N4F]	15.2
24.465	24.823	27.377	1,098.001	4+	1,196.238	3+	Y[pH4N3]	14.0
24.465	24.823	27.377	1,098.001	4+	1,317.948	3+	Y[pH5N4]	11.7
24.465	24.823	27.377	1,098.001	4+	1,339.696	2+	Y[pNF]*	10.9
24.465	24.823	27.377	1,098.001	4+	1,266.667	2+	Y[pN]	9.1
24.465	24.823	27.377	1,098.001	4+	1,530.260	2+	Y[pH2N2]	8.3
24.465	24.823	27.377	1,098.001	4+	1,611.286	2+	Y[pH3N2]	8.1
24.465	24.823	27.377	1,098.001	4+	1,866.881	2+	Y[pH4N3F]	7.8
24.465	24.823	27.377	1,098.001	4+	1,025.228	4+	Y[pH5N4F]	7.7
24.465	24.823	27.377	1,098.001	4+	1,142.220	3+	Y[pH3N3]	7.2
24.465	24.823	27.377	1,098.001	4+	1,785.855	2+	Y[pH3N3F]	7.1
24.465	24.823	27.377	1,098.001	4+	1,341.955	3+	Y[pH4N3FS]*	6.0

Spectra

4295;EThcD;24.441;4293;1098.005;Byonic:q-value=0.00589/GPS:MinICScore=3.533
4297;HCD;24.465;4293;1098.005;