TG004714

Peptide	R.TLYQFQFQEALCQAAKHEGPLHKCDISN[H3N3F]STEAGQK.L (PE001773)
Sequence	TLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQK
Glycans(s)	H3N3F (N28)
Mod(s)	57.021 (C12), 57.021 (C24)
Molecular Weight	5,293.37
Charge	5+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin_2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
35.128	35.356	53.944	1,056.085	5+	1,228.571	4+	Y[pH2N2F]	70.2
35.128	35.356	53.944	1,056.085	5+	1,269.084	4+	Y[pH3N2F]	61.4
35.128	35.356	53.944	1,056.085	5+	1,192.056	4+	Y[pH2N2]	31.0
35.128	35.356	53.944	1,056.085	5+	1,413.344	3+	Y[pN]	28.8
35.128	35.356	53.944	1,056.085	5+	1,232.570	4+	Y[pH3N2]	21.0
35.128	35.356	53.944	1,056.085	5+	1,151.543	4+	Y[pHN2]	16.9
35.128	35.356	53.944	1,056.085	5+	1,188.058	4+	Y[pHN2F]	16.6
35.128	35.356	53.944	1,056.085	5+	1,462.030	3+	Y[pNF]	13.5
35.128	35.356	53.944	1,056.085	5+	1,060.260	4+	Y[pN]	13.1
35.128	35.356	53.944	1,056.085	5+	1,111.030	4+	Y[pN2]	10.8
35.128	35.356	53.944	1,056.085	5+	1,096.775	4+	Y[pNF]	10.3
35.128	35.356	53.944	1,056.085	5+	1,147.545	4+	Y[pN2F]	9.3
35.128	35.356	53.944	1,056.085	5+	1,589.073	3+	Y[pH2N2]	6.4
35.128	35.356	53.944	1,056.085	5+	1,481.037	3+	Y[pN2]	5.2
35.128	35.356	53.944	1,056.085	5+	1,535.055	3+	Y[pHN2]	5.3

Spectra

6612;EThcD;34.979;6602;1056.286;Byonic:q-value=0.00656/GPS:MinICScore=51.588
6614;HCD;34.988;6602;1056.286;
6644;EThcD;35.120;6638;1056.085;Byonic:q-value=6.91e-05/GPS:MinICScore=55.672
6646;HCD;35.128;6638;1056.085;
6745;EThcD;35.560;6740;1056.085;Byonic:q-value=0.00106/GPS:MinICScore=21.549
6746;HCD;35.566;6740;1056.085;