TG004713

Peptide	R.TLYQFQFQEALCQAAKHEGPLHKCDISN[H3N4F]STEAGQK.L (PE001772)
Sequence	TLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQK
Glycans(s)	H3N4F (N28)
Mod(s)	57.021 (C12), 57.021 (C24)
Molecular Weight	5,496.45
Charge	5+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin_2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
35.304	35.282	53.756	1,096.701	5+	1,319.854	4+	Y[pH3N3F]	45.3
35.304	35.282	53.756	1,096.701	5+	1,279.341	4+	Y[pH2N3F]	31.0
35.304	35.282	53.756	1,096.701	5+	1,637.759	3+	Y[pH2N2F]*	15.3
35.304	35.282	53.756	1,096.701	5+	1,589.073	3+	Y[pH2N2]	12.6
35.304	35.282	53.756	1,096.701	5+	1,413.344	3+	Y[pN]	12.4
35.304	35.282	53.756	1,096.701	5+	1,283.340	4+	Y[pH3N3]	10.2
35.304	35.282	53.756	1,096.701	5+	1,242.826	4+	Y[pH2N3]	9.6
35.304	35.282	53.756	1,096.701	5+	1,535.055	3+	Y[pHN2]	9.6
35.304	35.282	53.756	1,096.701	5+	1,096.775	4+	Y[pNF]*	8.3
35.304	35.282	53.756	1,096.701	5+	1,462.030	3+	Y[pNF]*	8.1
35.304	35.282	53.756	1,096.701	5+	1,228.571	4+	Y[pH2N2F]*	7.8
35.304	35.282	53.756	1,096.701	5+	1,583.741	3+	Y[pHN2F]*	7.5
35.304	35.282	53.756	1,096.701	5+	1,691.776	3+	Y[pH3N2F]*	7.3
35.304	35.282	53.756	1,096.701	5+	1,192.056	4+	Y[pH2N2]	5.5
35.304	35.282	53.756	1,096.701	5+	1,060.260	4+	Y[pN]	5.1

Spectra

6772;EThcD;35.683;6767;1096.902;Byonic:q-value=0.00202/GPS:MinICScore=16.771
6774;HCD;35.691;6767;1096.902;