TG004670

Peptide	R.TLYQFQFQEALCQAAKHEGPLHKCDISN[H5N4FS]STEAGQK.L (PE001770)
Sequence	TLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQK
Glycans(s)	H5N4FS (N28)
Mod(s)	57.021 (C12), 57.021 (C24)
Molecular Weight	6,111.651
Charge	6+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin_2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
37.493	37.481	59.337	1,016.619	6+	1,269.084	4+	Y[pH3N2F]*	29.8
37.493	37.481	59.337	1,016.619	6+	1,413.344	3+	Y[pN]	20.1
37.493	37.481	59.337	1,016.619	6+	1,360.367	4+	Y[pH4N3F]	18.3
37.493	37.481	59.337	1,016.619	6+	1,192.056	4+	Y[pH2N2]	17.4
37.493	37.481	59.337	1,016.619	6+	1,319.854	4+	Y[pH3N3F]	17.3
37.493	37.481	59.337	1,016.619	6+	1,232.570	4+	Y[pH3N2]	13.0
37.493	37.481	59.337	1,016.619	6+	1,637.759	3+	Y[pH2N2F]*	10.9
37.493	37.481	59.337	1,016.619	6+	1,151.543	4+	Y[pHN2]	10.1
37.493	37.481	59.337	1,016.619	6+	1,060.260	4+	Y[pN]	10.0
37.493	37.481	59.337	1,016.619	6+	1,462.030	3+	Y[pNF]*	9.7
37.493	37.481	59.337	1,016.619	6+	1,589.073	3+	Y[pH2N2]	9.6
37.493	37.481	59.337	1,016.619	6+	1,096.701	5+	Y[pH3N4F]	8.7
37.493	37.481	59.337	1,016.619	6+	1,096.775	4+	Y[pNF]*	8.7
37.493	37.481	59.337	1,016.619	6+	1,188.058	4+	Y[pHN2F]*	8.2
37.493	37.481	59.337	1,016.619	6+	1,535.055	3+	Y[pHN2]	7.8
37.493	37.481	59.337	1,016.619	6+	1,283.340	4+	Y[pH3N3]	6.8
37.493	37.481	59.337	1,016.619	6+	1,187.330	5+	Y[pH4N4FS]	6.1
37.493	37.481	59.337	1,016.619	6+	1,111.030	4+	Y[pN2]	6.0
37.493	37.481	59.337	1,016.619	6+	1,583.741	3+	Y[pHN2F]*	5.7
37.493	37.481	59.337	1,016.619	6+	1,643.090	3+	Y[pH3N2]	5.5
37.493	37.481	59.337	1,016.619	6+	1,481.037	3+	Y[pN2]	5.3
37.493	37.481	59.337	1,016.619	6+	1,323.853	4+	Y[pH4N3]	5.1
37.493	37.481	59.337	1,016.619	6+	1,691.776	3+	Y[pH3N2F]*	8.2

Spectra

7148;EThcD;37.341;7147;1016.620;Byonic:q-value=0.00284/GPS:MinICScore=45.000
7150;HCD;37.349;7147;1016.620;
7180;EThcD;37.484;7178;1016.786;Byonic:q-value=0.00202/GPS:MinICScore=84.506
7182;HCD;37.493;7178;1016.786;