TG004668

Peptide	K.LQLQALQQN[H5N4F]GSSVLSEDKSKR.L (PE001739)
Sequence	LQLQALQQNGSSVLSEDKSKR
Glycans(s)	H5N4F (N9)
Mod(s)
Molecular Weight	4,114.89
Charge	4+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin_2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
21.384	21.061	17.665	1,025.228	4+	1,244.924	3+	Y[pH4N3F]	100.0
21.384	21.061	17.665	1,025.228	4+	1,190.906	3+	Y[pH3N3F]	35.4
21.384	21.061	17.665	1,025.228	4+	1,196.238	3+	Y[pH4N3]	14.6
21.384	21.061	17.665	1,025.228	4+	1,603.289	2+	Y[pH2N2F]*	16.4
21.384	21.061	17.665	1,025.228	4+	1,684.315	2+	Y[pH3N2F]*	16.2
21.384	21.061	17.665	1,025.228	4+	1,266.667	2+	Y[pN]	9.3
21.384	21.061	17.665	1,025.228	4+	1,530.260	2+	Y[pH2N2]	10.4
21.384	21.061	17.665	1,025.228	4+	1,142.220	3+	Y[pH3N3]	10.2
21.384	21.061	17.665	1,025.228	4+	1,611.286	2+	Y[pH3N2]	7.4
21.384	21.061	17.665	1,025.228	4+	1,339.696	2+	Y[pNF]*	9.6
21.384	21.061	17.665	1,025.228	4+	1,312.617	3+	Y[pH4N4F]	8.1
21.384	21.061	17.665	1,025.228	4+	1,522.262	2+	Y[pHN2F]*	5.3

Spectra

3671;EThcD;20.762;3670;1025.233;Byonic:q-value=4.93e-05/GPS:MinICScore=4.916
3673;HCD;20.771;3670;1025.233;
3810;EThcD;21.376;3806;1025.233;Byonic:q-value=1.01e-07/GPS:MinICScore=0.982
3812;HCD;21.384;3806;1025.233;
3903;EThcD;21.792;3894;1025.233;Byonic:q-value=0.000101/GPS:MinICScore=4.306
3904;HCD;21.797;3894;1025.233;