TG004643

Peptide	K.LQLQALQQN[H5N4FS2]GSSVLSEDKSK.R (PE001731)
Sequence	LQLQALQQNGSSVLSEDKSK
Glycans(s)	H5N4FS2 (N9)
Mod(s)
Molecular Weight	4,540.98
Charge	4+
Protein	ACE2 (Q9BYF1)
Spectrum File	MS_50_ACE2_Trypsin_2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	Human ACE2 Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
34.889	34.938	52.883	1,131.750	4+	1,606.265	2+	Y[pH3N2F]*	15.6
34.889	34.938	52.883	1,131.750	4+	1,188.617	2+	Y[pN]	15.0
34.889	34.938	52.883	1,131.750	4+	1,525.238	2+	Y[pH2N2F]*	14.6
34.889	34.938	52.883	1,131.750	4+	1,452.209	2+	Y[pH2N2]	11.6
34.889	34.938	52.883	1,131.750	4+	1,788.831	2+	Y[pH4N3F]	10.9
34.889	34.938	52.883	1,131.750	4+	1,707.804	2+	Y[pH3N3F]	10.9
34.889	34.938	52.883	1,131.750	4+	1,261.646	2+	Y[pNF]*	10.8
34.889	34.938	52.883	1,131.750	4+	1,289.922	3+	Y[pH4N3FS]	10.4
34.889	34.938	52.883	1,131.750	4+	1,533.236	2+	Y[pH3N2]	10.2
34.889	34.938	52.883	1,131.750	4+	1,192.890	3+	Y[pH4N3F]	9.4
34.889	34.938	52.883	1,131.750	4+	1,634.775	2+	Y[pH3N3]	8.2
34.889	34.938	52.883	1,131.750	4+	1,411.632	3+	Y[pH5N4FS]	8.0
34.889	34.938	52.883	1,131.750	4+	1,314.601	3+	Y[pH5N4F]	7.1
34.889	34.938	52.883	1,131.750	4+	1,715.802	2+	Y[pH4N3]	6.6

Spectra

6516;EThcD;34.552;6515;1131.760;Byonic:q-value=2.79e-05/GPS:MinICScore=14.025
6518;HCD;34.562;6515;1131.760;
6665;EThcD;35.207;6664;1131.757;Byonic:q-value=2.46e-05/GPS:MinICScore=3.849
6667;HCD;35.216;6664;1131.757;