TG004365

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Peptide R.EGVFVSN[H5N3F]GTHWFVTQR.N (PE001649)
Sequence EGVFVSNGTHWFVTQR
Glycans(s) H5N3F (N7)
Mod(s)
Molecular Weight 3,446.477
Charge 3+
Protein S (P0DTC2)
Spectrum File MS_50_Covid_spike_trypsin_new
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction No enrichment
Sample SARS-CoV-2 Spike Protein

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
32.242 32.269 45.810 1,143.830 3+ 1,532.675 2+ Y[pH4N2F] 75.1
32.242 32.269 45.810 1,143.830 3+ 1,451.649 2+ Y[pH3N2F] 41.2
32.242 32.269 45.810 1,143.830 3+ 1,459.646 2+ Y[pH4N2] 30.5
32.242 32.269 45.810 1,143.830 3+ 1,378.620 2+ Y[pH3N2] 25.0
32.242 32.269 45.810 1,143.830 3+ 1,613.701 2+ Y[pH5N2F]* 22.1
32.242 32.269 45.810 1,143.830 3+ 1,034.001 2+ Y[pN] 17.3
32.242 32.269 45.810 1,143.830 3+ 1,297.593 2+ Y[pH2N2] 16.5
32.242 32.269 45.810 1,143.830 3+ 1,370.622 2+ Y[pH2N2F] 14.3
32.242 32.269 45.810 1,143.830 3+ 1,107.030 2+ Y[pNF] 11.3
32.242 32.269 45.810 1,143.830 3+ 1,216.567 2+ Y[pHN2] 8.3
32.242 32.269 45.810 1,143.830 3+ 1,135.540 2+ Y[pN2] 7.8
32.242 32.269 45.810 1,143.830 3+ 1,289.596 2+ Y[pHN2F] 6.8
32.242 32.269 45.810 1,143.830 3+ 1,208.569 2+ Y[pN2F] 6.0
32.242 32.269 45.810 1,143.830 3+ 1,540.672 2+ Y[pH5N2]* 5.4
32.242 32.269 45.810 1,143.830 3+ 1,089.812 3+ Y[pH4N3F] 5.0

Spectra

6295;EThcD;32.174;6288;1143.838;Byonic:q-value=0.000176/GPS:MinICScore=13.792
6297;HCD;32.183;6288;1143.838;
6320;EThcD;32.292;6317;1144.171;Byonic:q-value=0.00104/GPS:MinICScore=8.665
6322;HCD;32.302;6317;1144.171;