TG004361

Peptide	R.EGVFVSN[H6N6FS]GTHWFVTQR.N (PE001651)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N6FS (N7)
Mod(s)
Molecular Weight	4,508.863
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
36.792	36.627	56.655	1,123.721	4+	1,735.754	2+	Y[pH4N4F]	23.3
36.792	36.627	56.655	1,123.721	4+	1,654.728	2+	Y[pH3N4F]	10.9
36.792	36.627	56.655	1,123.721	4+	1,472.162	2+	Y[pH2N3F]	8.5
36.792	36.627	56.655	1,123.721	4+	1,553.188	2+	Y[pH3N3F]	8.2
36.792	36.627	56.655	1,123.721	4+	1,662.725	2+	Y[pH4N4]	7.3
36.792	36.627	56.655	1,123.721	4+	1,918.320	2+	Y[pH5N5F]	6.9
36.792	36.627	56.655	1,123.721	4+	1,581.699	2+	Y[pH3N4]	5.5
36.792	36.627	56.655	1,123.721	4+	1,034.001	2+	Y[pN]	5.3
36.792	36.627	56.655	1,123.721	4+	1,816.781	2+	Y[pH5N4F]	5.2

Spectra

7256;EThcD;36.637;7253;1123.980;Byonic:q-value=4.02e-05/GPS:MinICScore=58.661
7258;HCD;36.646;7253;1123.980;
7317;EThcD;36.929;7310;1123.731;Byonic:q-value=9.67e-05/GPS:MinICScore=22.512
7319;HCD;36.937;7310;1123.731;