TG004358

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Peptide K.VCEFQFCNDPFLGVYYHKNN[H4N5F2S]K.S (PE001663)
Sequence VCEFQFCNDPFLGVYYHKNNK
Glycans(s) H4N5F2S (N20)
Mod(s) 57.021 (C2), 57.021 (C7)
Molecular Weight 4,943.035
Charge 4+
Protein S (P0DTC2)
Spectrum File MS_50_Covid_spike_trypsin_new
Method Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction No enrichment
Sample SARS-CoV-2 Spike Protein

Transitions

Exp. R.T. Peak R.T. Norm. R.T. Prec. m/z Prec. z Prod. m/z Prod. z Label %Int
42.985 42.768 71.938 1,232.270 4+ 1,361.587 3+ Y[pH4N3F] 15.9
42.985 42.768 71.938 1,232.270 4+ 1,458.619 3+ Y[pH4N3FS] 13.7
42.985 42.768 71.938 1,232.270 4+ 1,429.280 3+ Y[pH4N4F] 7.2
42.985 42.768 71.938 1,232.270 4+ 1,526.312 3+ Y[pH4N4FS] 6.3
42.985 42.768 71.938 1,232.270 4+ 1,859.310 2+ Y[pH3N2F]* 6.0
42.985 42.768 71.938 1,232.270 4+ 1,778.284 2+ Y[pH2N2F]* 5.1
42.985 42.768 71.938 1,232.270 4+ 1,307.569 3+ Y[pH3N3F] 5.0

Spectra

8628;EThcD;42.976;8618;1232.273;Byonic:q-value=0.000212/GPS:MinICScore=5.646
8630;HCD;42.985;8618;1232.273;