TG004357

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H5N3F]FTTAPAICHDGK.A (PE001652)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H5N3F (N29)
Mod(s)	57.021 (C37)
Molecular Weight	6,134.796
Charge	6+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
40.067	39.785	64.514	1,020.474	6+	1,151.341	5+	Y[pH4N2F]	42.4
40.067	39.785	64.514	1,020.474	6+	1,021.699	5+	Y[pN2F]	33.4
40.067	39.785	64.514	1,020.474	6+	1,118.930	5+	Y[pH3N2F]	25.6
40.067	39.785	64.514	1,020.474	6+	1,189.587	4+	Y[pN]	18.2
40.067	39.785	64.514	1,020.474	6+	1,089.719	5+	Y[pH3N2]	14.3
40.067	39.785	64.514	1,020.474	6+	993.465	6+	Y[pH4N3F]	12.7
40.067	39.785	64.514	1,020.474	6+	1,226.102	4+	Y[pNF]	10.7
40.067	39.785	64.514	1,020.474	6+	1,122.129	5+	Y[pH4N2]	10.1
40.067	39.785	64.514	1,020.474	6+	951.871	5+	Y[pN]	7.3
40.067	39.785	64.514	1,020.474	6+	992.487	5+	Y[pN2]	7.0
40.067	39.785	64.514	1,020.474	6+	1,183.752	5+	Y[pH5N2F]*	5.8

Spectra

7989;EThcD;40.065;7984;1020.479;Byonic:q-value=0.00104/GPS:MinICScore=82.655
7991;HCD;40.073;7984;1020.479;