TG004354

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H7N6FS]FTTAPAICHDGK.A (PE001656)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H7N6FS (N29)
Mod(s)	57.021 (C37)
Molecular Weight	7,359.236
Charge	6+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
42.342			1,224.547	6+	1,176.032	6+	Y[pH7N6F]	35.2
42.342			1,224.547	6+	1,338.010	5+	Y[pH6N5F]	23.4
42.342			1,224.547	6+	1,226.102	4+	Y[pNF]*	12.3
42.342			1,224.547	6+	1,396.229	5+	Y[pH6N5FS]	10.4
42.342			1,224.547	6+	1,580.977	4+	Y[pH5N4F]	9.4
42.342			1,224.547	6+	1,449.181	4+	Y[pH3N3F]	6.8
42.342			1,224.547	6+	1,232.573	5+	Y[pH4N4F]	5.4

Spectra

8285;EThcD;41.430;8275;1224.553;Byonic:q-value=1.42e-10/GPS:MinICScore=31.826
8287;HCD;41.438;8275;1224.553;
8862;EThcD;44.058;8857;1224.548;Byonic:q-value=2.01e-08/GPS:MinICScore=69.953
8864;HCD;44.067;8857;1224.548;
9243;EThcD;45.868;9234;1224.553;Byonic:q-value=0.000257/GPS:MinICScore=87.475
9245;HCD;45.876;9234;1224.553;