TG004353

Peptide	R.DLPQGFSALEPLVDLPIGIN[H5N3F]ITR.F (PE001647)
Sequence	DLPQGFSALEPLVDLPIGINITR
Glycans(s)	H5N3F (N20)
Mod(s)
Molecular Weight	4,060.924
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
72.295	72.600	147.440	1,011.736	4+	1,341.224	2+	Y[pN]	76.3
72.295	72.600	147.440	1,011.736	4+	1,010.864	3+	Y[pN2F]	12.5
72.295	72.600	147.440	1,011.736	4+	1,186.593	3+	Y[pH2N3F]	9.3
72.295	72.600	147.440	1,011.736	4+	1,414.253	2+	Y[pNF]	7.3
72.295	72.600	147.440	1,011.736	4+	1,070.214	3+	Y[pH2N2]	7.1
72.295	72.600	147.440	1,011.736	4+	826.792	3+	Y[p]	6.8
72.295	72.600	147.440	1,011.736	4+	1,239.684	2+	Y[p]	5.5
72.295	72.600	147.440	1,011.736	4+	894.485	3+	Y[pN]	5.1

Spectra

14874;EThcD;72.285;14872;1011.991;Byonic:q-value=0.000751/GPS:MinICScore=171.956
14876;HCD;72.295;14872;1011.991;