TG004339

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H5N3F]FTTAPAICHDGK.A (PE001652)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H5N3F (N29)
Mod(s)	57.021 (C37)
Molecular Weight	6,134.796
Charge	7+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
39.225			874.836	7+	959.619	6+	Y[pH4N2F]	85.5
39.225			874.836	7+	951.871	5+	Y[pN]	59.5
39.225			874.836	7+	932.610	6+	Y[pH3N2F]	40.0
39.225			874.836	7+	981.083	5+	Y[pNF]	25.9
39.225			874.836	7+	1,189.587	4+	Y[pN]	18.2
39.225			874.836	7+	905.601	6+	Y[pH2N2F]	17.7
39.225			874.836	7+	935.276	6+	Y[pH4N2]	14.5
39.225			874.836	7+	992.487	5+	Y[pN2]	11.7
39.225			874.836	7+	854.249	6+	Y[pHN2]	11.7
39.225			874.836	7+	827.241	6+	Y[pN2]	9.5
39.225			874.836	7+	908.267	6+	Y[pH3N2]	12.7
39.225			874.836	7+	817.737	6+	Y[pNF]	9.5
39.225			874.836	7+	1,089.719	5+	Y[pH3N2]	8.5
39.225			874.836	7+	853.971	7+	Y[pH5N3]	9.2
39.225			874.836	7+	793.394	6+	Y[pN]	6.7
39.225			874.836	7+	878.592	6+	Y[pHN2F]	6.0
39.225			874.836	7+	911.255	5+	Y[p]	5.1
39.225			874.836	7+	851.584	6+	Y[pN2F]	5.2
39.225			874.836	7+	1,054.109	5+	Y[pHN2F]	5.5

Spectra

7596;EThcD;38.876;7595;874.840;Byonic:q-value=2.69e-07/GPS:MinICScore=80.421
7598;HCD;38.885;7595;874.840;
7649;EThcD;39.104;7647;874.982;Byonic:q-value=3.09e-07/GPS:MinICScore=92.606
7651;HCD;39.114;7647;874.982;
7675;EThcD;39.215;7673;875.126;Byonic:q-value=2.77e-07/GPS:MinICScore=77.086
7677;HCD;39.225;7673;875.126;
7792;EThcD;39.727;7787;874.982;Byonic:q-value=3.6e-05/GPS:MinICScore=59.701
7794;HCD;39.735;7787;874.982;