TG004337

Peptide	R.EGVFVSN[H6N7]GTHWFVTQR.N (PE001650)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N7 (N7)
Mod(s)
Molecular Weight	4,274.789
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new2
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
30.895	31.043	42.912	1,065.203	4+	1,764.265	2+	Y[pH4N5]	20.3
30.895	31.043	42.912	1,065.203	4+	1,845.292	2+	Y[pH5N5]	7.6
30.895	31.043	42.912	1,065.203	4+	1,581.699	2+	Y[pH3N4]	8.1
30.895	31.043	42.912	1,065.203	4+	1,662.725	2+	Y[pH4N4]	6.7
30.895	31.043	42.912	1,065.203	4+	1,034.001	2+	Y[pN]	6.4
30.895	31.043	42.912	1,065.203	4+	1,480.159	2+	Y[pH3N3]	5.1
30.895	31.043	42.912	1,065.203	4+	1,500.673	2+	Y[pH2N4]	5.4
30.895	31.043	42.912	1,065.203	4+	1,298.223	3+	Y[pH5N6]	5.3

Spectra

5886;EThcD;30.821;5885;1065.460;Byonic:q-value=2.09e-05/GPS:MinICScore=54.934
5888;HCD;30.830;5885;1065.460;
5900;EThcD;30.885;5899;1065.711;Byonic:q-value=2.84e-05/GPS:MinICScore=124.848
5902;HCD;30.895;5899;1065.711;
5913;EThcD;30.945;5910;1065.209;Byonic:q-value=4.45e-05/GPS:MinICScore=48.217
5915;HCD;30.954;5910;1065.209;