TG004309

Peptide	R.EGVFVSN[H4N4]GTHWFVTQR.N (PE001530)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H4N4 (N7)
Mod(s)
Molecular Weight	3,341.446
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.617	32.560	46.534	1,108.820	3+	1,480.159	2+	Y[pH3N3]	71.1
32.617	32.560	46.534	1,108.820	3+	1,399.133	2+	Y[pH2N3]	28.1
32.617	32.560	46.534	1,108.820	3+	1,378.620	2+	Y[pH3N2]	20.6
32.617	32.560	46.534	1,108.820	3+	1,297.593	2+	Y[pH2N2]	17.0
32.617	32.560	46.534	1,108.820	3+	1,034.001	2+	Y[pN]	16.4
32.617	32.560	46.534	1,108.820	3+	1,135.540	2+	Y[pN2]	10.1
32.617	32.560	46.534	1,108.820	3+	1,216.567	2+	Y[pHN2]	9.6
32.617	32.560	46.534	1,108.820	3+	1,459.646	2+	Y[pH4N2]*	6.0

Spectra

6387;EThcD;32.608;6381;1108.827;Byonic:q-value=0.00104/GPS:MinICScore=31.966
6389;HCD;32.617;6381;1108.827;