TG004295

Peptide	K.VCEFQFCNDPFLGVYYHKNN[H4N5F2]K.S (PE001632)
Sequence	VCEFQFCNDPFLGVYYHKNNK
Glycans(s)	H4N5F2 (N20)
Mod(s)	57.021 (C2), 57.021 (C7)
Molecular Weight	4,651.939
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
38.378	38.332	60.898	1,159.496	4+	1,361.587	3+	Y[pH4N3F]	24.8
38.378	38.332	60.898	1,159.496	4+	1,429.280	3+	Y[pH4N4F]	12.2
38.378	38.332	60.898	1,159.496	4+	1,307.569	3+	Y[pH3N3F]	7.2
38.378	38.332	60.898	1,159.496	4+	1,410.273	3+	Y[pH4N3F2]*	6.7
38.378	38.332	60.898	1,159.496	4+	1,477.966	3+	Y[pH4N4F2]	6.0
38.378	38.332	60.898	1,159.496	4+	1,859.310	2+	Y[pH3N2F]	5.9
38.378	38.332	60.898	1,159.496	4+	1,778.284	2+	Y[pH2N2F]	5.3
38.378	38.332	60.898	1,159.496	4+	1,514.691	2+	Y[pNF]	5.0

Spectra

7578;EThcD;38.151;7577;1159.500;Byonic:q-value=1.57e-06/GPS:MinICScore=3.697
7580;HCD;38.160;7577;1159.500;
7665;EThcD;38.538;7662;1159.500;Byonic:q-value=0.000113/GPS:MinICScore=10.528
7667;HCD;38.596;7662;1159.500;