TG004293

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H6N5F]FTTAPAICHDGK.A (PE001548)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H6N5F (N29)
Mod(s)	57.021 (C37)
Molecular Weight	6,703.008
Charge	6+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
40.141	40.538	66.388	1,115.176	6+	1,264.983	5+	Y[pH5N4F]	33.8
40.141	40.538	66.388	1,115.176	6+	1,159.546	5+	Y[pH3N3F]	9.4
40.141	40.538	66.388	1,115.176	6+	1,449.181	4+	Y[pH3N3F]	7.3
40.141	40.538	66.388	1,115.176	6+	1,232.573	5+	Y[pH4N4F]	6.9
40.141	40.538	66.388	1,115.176	6+	1,189.587	4+	Y[pN]	6.0
40.141	40.538	66.388	1,115.176	6+	1,357.898	4+	Y[pH2N2F]*	5.2
40.141	40.538	66.388	1,115.176	6+	1,321.383	4+	Y[pH2N2]	5.2
40.141	40.538	66.388	1,115.176	6+	1,088.167	6+	Y[pH5N5F]	5.0

Spectra

7981;EThcD;40.032;7971;1115.185;Byonic:q-value=0.00346/GPS:MinICScore=28.513
7983;HCD;40.040;7971;1115.185;
8026;EThcD;40.234;8021;1115.514;Byonic:q-value=0.00104/GPS:MinICScore=37.018
8028;HCD;40.242;8021;1115.514;