TG004282

Peptide	K.VCEFQFCNDPFLGVYYHKNN[H4N5FS]K.S (PE001636)
Sequence	VCEFQFCNDPFLGVYYHKNNK
Glycans(s)	H4N5FS (N20)
Mod(s)	57.021 (C2), 57.021 (C7)
Molecular Weight	4,796.977
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
43.179	42.947	72.384	1,195.756	4+	1,361.587	3+	Y[pH4N3F]	15.1
43.179	42.947	72.384	1,195.756	4+	1,429.280	3+	Y[pH4N4F]	13.8
43.179	42.947	72.384	1,195.756	4+	1,375.262	3+	Y[pH3N4F]	11.1
43.179	42.947	72.384	1,195.756	4+	1,526.312	3+	Y[pH4N4FS]	7.6
43.179	42.947	72.384	1,195.756	4+	1,496.973	3+	Y[pH4N5F]	7.2
43.179	42.947	72.384	1,195.756	4+	1,307.569	3+	Y[pH3N3F]	6.2
43.179	42.947	72.384	1,195.756	4+	1,859.310	2+	Y[pH3N2F]*	5.8
43.179	42.947	72.384	1,195.756	4+	1,458.619	3+	Y[pH4N3FS]	5.6
43.179	42.947	72.384	1,195.756	4+	1,514.691	2+	Y[pNF]*	5.3
43.179	42.947	72.384	1,195.756	4+	1,442.955	3+	Y[pH3N5F]	5.2
43.179	42.947	72.384	1,195.756	4+	1,778.284	2+	Y[pH2N2F]*	5.0

Spectra

8625;EThcD;42.963;8618;1195.759;Byonic:q-value=4.05e-07/GPS:MinICScore=13.687
8627;HCD;42.972;8618;1195.759;
8715;EThcD;43.378;8706;1195.759;Byonic:q-value=5.55e-05/GPS:MinICScore=23.642
8717;HCD;43.386;8706;1195.759;