TG004273

Peptide	K.VCEFQFCNDPFLGVYYHKNN[H3N4F]K.S (PE001580)
Sequence	VCEFQFCNDPFLGVYYHKNNK
Glycans(s)	H3N4F (N20)
Mod(s)	57.021 (C2), 57.021 (C7)
Molecular Weight	4,140.749
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
39.192	39.044	62.670	1,031.699	4+	1,307.569	3+	Y[pH3N3F]	49.2
39.192	39.044	62.670	1,031.699	4+	1,253.552	3+	Y[pH2N3F]	36.9
39.192	39.044	62.670	1,031.699	4+	1,441.662	2+	Y[pN]	14.4
39.192	39.044	62.670	1,031.699	4+	1,778.284	2+	Y[pH2N2F]*	13.9
39.192	39.044	62.670	1,031.699	4+	1,185.859	3+	Y[pH2N2F]*	10.7
39.192	39.044	62.670	1,031.699	4+	1,514.691	2+	Y[pNF]*	8.9
39.192	39.044	62.670	1,031.699	4+	1,697.257	2+	Y[pHN2F]*	7.6
39.192	39.044	62.670	1,031.699	4+	1,239.876	3+	Y[pH3N2F]*	7.0
39.192	39.044	62.670	1,031.699	4+	1,859.310	2+	Y[pH3N2F]*	6.8
39.192	39.044	62.670	1,031.699	4+	1,705.255	2+	Y[pH2N2]	6.6

Spectra

7794;EThcD;39.184;7788;1031.700;Byonic:q-value=0.000134/GPS:MinICScore=27.991
7796;HCD;39.192;7788;1031.700;