TG004270

Peptide	R.EGVFVSN[H6N3]GTHWFVTQR.N (PE001513)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H6N3 (N7)
Mod(s)
Molecular Weight	3,462.472
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
32.115	32.260	45.787	1,149.162	3+	1,540.672	2+	Y[pH5N2]	100.0
32.115	32.260	45.787	1,149.162	3+	1,459.646	2+	Y[pH4N2]	71.8
32.115	32.260	45.787	1,149.162	3+	1,378.620	2+	Y[pH3N2]	27.6
32.115	32.260	45.787	1,149.162	3+	1,034.001	2+	Y[pN]	27.4
32.115	32.260	45.787	1,149.162	3+	1,297.593	2+	Y[pH2N2]	20.6
32.115	32.260	45.787	1,149.162	3+	1,621.699	2+	Y[pH6N2]*	17.8
32.115	32.260	45.787	1,149.162	3+	1,135.540	2+	Y[pN2]	12.0
32.115	32.260	45.787	1,149.162	3+	1,216.567	2+	Y[pHN2]	11.2
32.115	32.260	45.787	1,149.162	3+	1,095.144	3+	Y[pH5N3]	5.7

Spectra

6280;EThcD;32.105;6278;1149.171;Byonic:q-value=0.00104/GPS:MinICScore=7.352
6282;HCD;32.115;6278;1149.171;