TG004268

Peptide	R.EGVFVSN[H5N4FS]GTHWFVTQR.N (PE001506)
Sequence	EGVFVSNGTHWFVTQR
Glycans(s)	H5N4FS (N7)
Mod(s)
Molecular Weight	3,940.652
Charge	3+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
37.127	37.311	58.357	1,308.555	3+	1,634.215	2+	Y[pH4N3F]	73.4
37.127	37.311	58.357	1,308.555	3+	1,553.188	2+	Y[pH3N3F]	36.6
37.127	37.311	58.357	1,308.555	3+	1,480.159	2+	Y[pH3N3]	11.9
37.127	37.311	58.357	1,308.555	3+	1,211.523	3+	Y[pH5N4F]	10.4
37.127	37.311	58.357	1,308.555	3+	1,816.781	2+	Y[pH5N4F]	9.9
37.127	37.311	58.357	1,308.555	3+	1,779.762	2+	Y[pH4N3FS]*	9.6
37.127	37.311	58.357	1,308.555	3+	1,451.649	2+	Y[pH3N2F]*	8.5
37.127	37.311	58.357	1,308.555	3+	1,370.622	2+	Y[pH2N2F]*	8.3
37.127	37.311	58.357	1,308.555	3+	1,107.030	2+	Y[pNF]*	8.2
37.127	37.311	58.357	1,308.555	3+	1,034.001	2+	Y[pN]	7.5
37.127	37.311	58.357	1,308.555	3+	1,297.593	2+	Y[pH2N2]	6.6
37.127	37.311	58.357	1,308.555	3+	1,735.754	2+	Y[pH4N4F]	6.3
37.127	37.311	58.357	1,308.555	3+	1,378.620	2+	Y[pH3N2]	6.2

Spectra

7325;EThcD;36.962;7320;1308.566;Byonic:q-value=0.00346/GPS:MinICScore=0.544
7326;HCD;36.969;7320;1308.566;
7438;EThcD;37.496;7427;1308.564;Byonic:q-value=0.00104/GPS:MinICScore=3.661
7440;HCD;37.505;7427;1308.564;