TG004264

Peptide	K.GYHLMSFPQSAPHGVVFLHVTYVPAQEKN[H6N6FS2]FTTAPAICHDGK.A (PE001557)
Sequence	GYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGK
Glycans(s)	H6N6FS2 (N29)
Mod(s)	57.021 (C37)
Molecular Weight	7,488.278
Charge	7+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
44.000	43.969	74.927	1,068.191	7+	1,136.683	6+	Y[pH5N5FS]	17.7
44.000	43.969	74.927	1,068.191	7+	1,197.539	6+	Y[pH6N6FS]	14.0
44.000	43.969	74.927	1,068.191	7+	1,149.023	6+	Y[pH6N6F]	14.0
44.000	43.969	74.927	1,068.191	7+	1,189.587	4+	Y[pN]	11.6
44.000	43.969	74.927	1,068.191	7+	1,240.357	4+	Y[pN2]	9.7
44.000	43.969	74.927	1,068.191	7+	1,232.573	5+	Y[pH4N4F]	7.4
44.000	43.969	74.927	1,068.191	7+	1,118.930	5+	Y[pH3N2F]*	7.2
44.000	43.969	74.927	1,068.191	7+	1,151.353	6+	Y[pH5N4FS2]	6.7
44.000	43.969	74.927	1,068.191	7+	1,122.014	6+	Y[pH5N6F]	6.6
44.000	43.969	74.927	1,068.191	7+	1,026.606	7+	Y[pH6N6FS]	6.5
44.000	43.969	74.927	1,068.191	7+	1,226.102	4+	Y[pNF]*	6.2
44.000	43.969	74.927	1,068.191	7+	1,191.957	5+	Y[pH4N3F]	5.8
44.000	43.969	74.927	1,068.191	7+	951.871	5+	Y[pN]	5.7
44.000	43.969	74.927	1,068.191	7+	1,408.668	4+	Y[pH2N3F]	5.6
44.000	43.969	74.927	1,068.191	7+	1,449.181	4+	Y[pH3N3F]	5.6
44.000	43.969	74.927	1,068.191	7+	1,357.898	4+	Y[pH2N2F]*	5.4
44.000	43.969	74.927	1,068.191	7+	1,159.546	5+	Y[pH3N3F]	5.2
44.000	43.969	74.927	1,068.191	7+	1,305.599	5+	Y[pH5N5F]	5.1

Spectra

8846;EThcD;43.991;8844;1068.479;Byonic:q-value=0.00104/GPS:MinICScore=198.977
8848;HCD;44.000;8844;1068.479;