TG004245

Peptide	K.VCEFQFCNDPFLGVYYHKNN[H5N4F2]K.S (PE001583)
Sequence	VCEFQFCNDPFLGVYYHKNNK
Glycans(s)	H5N4F2 (N20)
Mod(s)	57.021 (C2), 57.021 (C7)
Molecular Weight	4,610.913
Charge	4+
Protein	S (P0DTC2)
Spectrum File	MS_50_Covid_spike_trypsin_new
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	No enrichment
Sample	SARS-CoV-2 Spike Protein

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
38.207	38.374	61.003	1,149.240	4+	1,361.587	3+	Y[pH4N3F]	21.5
38.207	38.374	61.003	1,149.240	4+	1,441.662	2+	Y[pN]	9.7
38.207	38.374	61.003	1,149.240	4+	1,307.569	3+	Y[pH3N3F]	8.1
38.207	38.374	61.003	1,149.240	4+	1,514.691	2+	Y[pNF]*	8.0
38.207	38.374	61.003	1,149.240	4+	1,410.273	3+	Y[pH4N3F2]*	7.1
38.207	38.374	61.003	1,149.240	4+	1,778.284	2+	Y[pH2N2F]*	5.5
38.207	38.374	61.003	1,149.240	4+	1,483.298	3+	Y[pH5N4F]	5.0

Spectra

7589;EThcD;38.199;7585;1149.241;Byonic:q-value=8.71e-05/GPS:MinICScore=5.001
7591;HCD;38.207;7585;1149.241;